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Effects of murine cytomegalovirus infection on sperm viability in mice.
Links Effects of murine cytomegalovirus infection on sperm viability in mice. Xiong J, Xiong C, Tian Y, Hu L, Wei HJ Huazhong Univ Sci Technolog Med Sci. 2006;26(1):130-2. Links Effects of murine cytomegalovirus infection on sperm viability in mice. Xiong J, Xiong C, Tian Y, Hu L, Wei H. Center of Reproductive Medicine, Research Institute of Family Planning, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. In order to explore the effects of testicular infection of murine cytomegalovirus (MCMV) on mature sperm viability at different periods following MCMV inoculation in mice, 91 BALB/c mice without MCMV infection were randomly divided into two groups: an experimental group (n = 56) and a control group (n = 35). The mice in the experimental group were treated by inoculating MCMV intratesticularly, while those in the controlled group were directly inoculated with DMEM without MCMV. The mice in both groups were sacrificed separately on the day 1, 1. 5, 2, 4, 6, 9 and 14 post-inoculation (D1) 1. 5, 2, 4, 6, 9 and 14 PI). The MCMV M83 mRNA gene was detected in the testis by in situ hybridization (ISH) with MCMV late-mRNA probe labeled with digoxin. Sperm viability of mature sperm in the epididymis cauda was measured. The results demonstrated the positive signal of ISH of MCMV was found mainly in the cytoplasm of the testicular interstitial cells and spermatogenic cells in the experimental group. Compared with that in the controlled group, the sperm viability in the experimental group was decreased significantly on D1 PI and D1.5 PI (P 0.05). This suggested that sperm viability in mice might be descended significantly shortly after MCMV infection and might return to normal with time, indicating that MCMV acute infection might temporarily degrade sperm quality and influence procreation transiently. PMID: 16711027 [PubMed - indexed for MEDLINE] Related Links [Effects of testis murine cytomegalovirus infection on sperm acrosome reaction and spermatic function of membrane in mice] [Zhonghua Yi Xue Za Zhi. 2005] PMID: 16251079 [Experimental study on effects of testis murine cytomegalovirus infection on sperm viability in mice] [Zhonghua Nan Ke Xue. 2005] PMID: 15999488 Biphasic viremia and viral gene expression in leukocytes during acute cytomegalovirus infection of mice. [J Virol. 1994] PMID: 8083970 The pathogenesis of murine cytomegalovirus ocular infection. Anterior chamber inoculation. [Invest Ophthalmol Vis Sci. 1990] PMID: 2167300 Cytomegalovirus infection of murine testicular interstitial Leydig cells. [Infect Immun. 1983] PMID: 6302003 See all Related Articles... Display Summary Brief Abstract AbstractPlus Citation MEDLINE XML UI List LinkOut ASN.1 Related Articles Cited Articles Cited in Books CancerChrom Links Domain Links 3D Domain Links GEO DataSet Links Gene Links Gene (GeneRIF) Links Genome Links Project Links GENSAT Links GEO Profile Links HomoloGene Links Nucleotide Links Nucleotide (RefSeq) Links OMIA Links OMIM (calculated) Links OMIM (cited) Links BioAssay Links Compound Links Compound via MeSH Substance Links Substance via MeSH PMC Links Cited in PMC PopSet Links Probe Links Protein Links Protein (RefSeq) Links SNP Links Structure Links Taxonomy via GenBank UniGene Links UniSTS Links Show 5 10 20 50 100 200 500 Sort by Pub Date First Author Last Author Journal Send to Text File Printer Clipboard E-mail Order .
Center of Reproductive Medicine, Research Institute of Family Planning, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
In order to explore the effects of testicular infection of murine cytomegalovirus (MCMV) on mature sperm viability at different periods following MCMV inoculation in mice, 91 BALB/c mice without MCMV infection were randomly divided into two groups: an experimental group (n = 56) and a control group (n = 35). The mice in the experimental group were treated by inoculating MCMV intratesticularly, while those in the controlled group were directly inoculated with DMEM without MCMV. The mice in both groups were sacrificed separately on the day 1, 1. 5, 2, 4, 6, 9 and 14 post-inoculation (D1) 1. 5, 2, 4, 6, 9 and 14 PI). The MCMV M83 mRNA gene was detected in the testis by in situ hybridization (ISH) with MCMV late-mRNA probe labeled with digoxin. Sperm viability of mature sperm in the epididymis cauda was measured. The results demonstrated the positive signal of ISH of MCMV was found mainly in the cytoplasm of the testicular interstitial cells and spermatogenic cells in the experimental group. Compared with that in the controlled group, the sperm viability in the experimental group was decreased significantly on D1 PI and D1.5 PI (P < 0.05). No statistically significant difference in the sperm viability was found after D2 PI between two groups (P > 0.05). This suggested that sperm viability in mice might be descended significantly shortly after MCMV infection and might return to normal with time, indicating that MCMV acute infection might temporarily degrade sperm quality and influence procreation transiently.
This abstract is being posted for educational purposes, as well as for comment and criticism, by the visitors to the Epididymitis Foundation website (EpididymitisFoundation.org). This abstract is representative of a larger article that is indexed on Medline.
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